Cell culture is a commonly used laboratory technique, where cells are grown under controlled conditions typically outside of their natural environment.
Cell culture is an important part in cellular and molecular biology, as well as within the pharmaceutical industry, as it is useful to study the effects of drugs and toxic compounds on cells. This characteristic ensures that cell culture also plays a vital part in the manufacture of viral vaccines drug screening and development. Studies into mutagenesis and carcinogenesis are also made possible by this technique.
Due to the critical requirement for accurate, reliable and reproducible results within these fields, cell cultures pose a major advantage for consistent results.
Contaminated cultures and cell death are a major problem that can ultimately impact on downstream experiments. Water quality plays an important role in cell culture experimental outcomes and is used throughout the cell culture process. Not only is it the main component of buffers and media, but it is used for dissolution of additives and drugs etc.
Apyrogenic ultrapure water (Type I) should be used for the preparation of media and buffers to ensure that cells are free from bacterial, yeast and viral contaminants. Contamination of cell culture by metal ions, endotoxins, bacteria or fungi can impact the culture directly or indirectly (e.g. changes in pH).
Make sure that you are using the right water type for your application. Here are the requirements for various cell culture applications.
|Bacteria (CFU/ml)||Endotoxins (EU/ml)||Nucleases||Water grade|
|Bacterial cell culture||General||>1||<50||<0.2||<1||NA||NA||II|
|Mammalian cell culture||High||>18||<10||Ultrafilter||<1||<0.002||Not Detected||I|
|Plant tissue culture||High||>18||<10||Ultrafilter||<1||<0.002||Not Detected||I|